斑马鱼，脊椎动物视网膜行为学和遗传学研究工具

Zebrafish:Atoolforbehavioralandgeneticstudiesofvertebrateretinalfunctionanddegeneration#XIYang,ZHANGHuafeng,HUANGTao,YEQijun,LIXinle,HENingning,LIU5101520253035Liping,SUNLei,FANGYangwu,SHAOJinping,CHENDongyan,LIYuhao,LILei**(KeyLaboratoryofAnimalModelsandDegenerativeNeurologicalDiseases,MedicalSchool,NankaiUniversity,Tianjin300071)Abstract:Invisionresearch,publicationsrelateto„visualsensitivity‟areoftenrestrictedtooneofthethreedimensions:absolutevisualsensitivity,contrastsensitivityandspectral/temporalsensitivity.Ourownresearchusingthezebrafishmodel(Daniorerio)ismainlyconcernedwithabsolutesensitivity,andtoalesserextent,contrastandspectral/temporalsensitivity.Inthispaper,wewillreviewtheusefulnessofzebrafishasamodelsystemforbehavioralandgeneticstudiesofabsolutevisualsensitivity.Keywords:animal;zebrafish;retina;mutation;behavioraltest;visualsensitivity0IntroductionZebrafishhasemergedasoneofthemajorgeneticmodelsystemsforstudyingvertebratevisualsystemfunctionsanddiseases.Similartohumans,thezebrafishretinaisanorganizedneuraltissue.Itconsistsofthreenucleilayers,whichincludeouternucleilayer,innernucleilayerandganglioncelllayer.Nucleilayersareseparatedbytwoplexiformlayers,namely,outer-plexiformlayerandinner-plexiformlayer.Photoreceptorcellsarelocatedintheouternucleilayer.Photoreceptorcelloutersegmentsareembeddedinretinalpigmentepithelia.Otherretinalcelltypes,suchasretinalhorizontalcells,bipolarcellsandamacrinecellsarefoundintheinnernucleilayer.Retinalganglioncellsarerestrictedtotheganglioncelllayer.Occasionally,asmallnumberofdisplacedganglioncellsarefoundintheinnernucleilayer.Neuralprocesses(i.e.,axonsandsynapses)betweendifferentretinalcelltypesaremainlyfoundintheouter-andinner-plexiformlayers[1,2].Similartoothervertebratespeciesincludinghumans,thezebrafishretinascontainbothrodandconephotoreceptorcells.Inzebrafish,onetypeofrodphotoreceptorcellsandfourtypesofconephotoreceptorcellshavebeenidentified.Rodphotoreceptorcellsmediatedim-lightandnightvision.Theyaredistributedallovertheretinas(dorsal,ventral,nasalandtemporalretinas,respective).Conephotoreceptorcells,incontrast,areresponsibleformediatingday-timebrightlightandcolorvision.Dependingontheexpressionofopsinproteinsandthemaximumabsorptionoflight-wavelength,conephotoreceptorcellscanbeclassifiedasLcones(longwavelengthsensitive),Mcones(middlewavelengthsensitive),Scones(shortwavelengthsensitive)andUVcones(ultravioletsensitive),respectively.Inzebrafish,LandMconesform“double-cones”.Foundations:SpecializedResearchFundfortheDoctoralProgramofHigherEducation(No.20090031110037),NationalScienceFoundation(No.81171066)andTianjinScience-TechCommittee(No.12JC***C24000).Briefauthorintroduction:XIYang(1987-),female,student,neuralbiologyCorrespondanceauthor:LILei(1968-),male,professor,neuralbiology.leili@nankai.edu.cn-1-

相关知识

斑马鱼，脊椎动物视网膜行为学和遗传学研究工具
斑马鱼行为篇①：斑马鱼嗅觉行为学和简单运动行为学研究
斑马鱼行为篇㉚：浅析斑马鱼捕食和避害行为
脑声笔记︱斑马鱼行为篇①：斑马鱼嗅觉行为学和简单运动行为学研究
斑马鱼行为篇：斑马鱼在心血管疾病中应用
斑马鱼行为篇㊻：斑马鱼模型在糖尿病研究中的应用
光遗传学技术应用于动物行为学研究的新进展
斑马鱼行为篇：药源性眼损伤斑马鱼模型
斑马鱼行为篇㊶：斑马鱼在听觉领域的应用
斑马鱼行为篇㉘：浅析斑马鱼孤独症模型

网址: 斑马鱼，脊椎动物视网膜行为学和遗传学研究工具 https://www.mcbbbk.com/newsview505783.html